Direct comparison of KATUSHKA far-red fluorescent protein variants with luciferase bioluminescence in xenograft mouse models of epithelial tumor studies

Background. Research in oncology assumes establishment and usage of xenograft animal models, meeting the requirements humanity rationality. Although praised as promising field research, preclinical studies low-molecular-weight inhibitors, antitumor monoclonal antibodies, vaccines, cellular therapy products, CAR-T cells entail continuous control tumor growth dynamics. Luciferase detection bioluminescence requires injection a reporter substrate. However, injections not only are laborious, time-consuming expensive, but also stressful for animals. Thus, vast variety new visualization methods is employed, including proteins far-red spectrum. Objective. The study aimed to compare efficiency dynamics mice models cervical cancer, applying commercially available line fluorescent red protein derivatives Katushka clade Renilla luciferase, that commonly used vivo . Design methods. Xenograft were derived injecting modified HeLa cell line, expresses proteins: Katushka, Katushka2S, TurboRFP, TurboFP650 enzyme. Results. Spectral properties emission wavelength Katushka2S outlines these markers within RFP lineage outstanding instrument visualization. Conclusion. Detection reporters can be considered credible alternative luciferase experimental on part immunotherapy research.